[ genotoxic effects of mobile phone waves on induction of chromosomal damages in embryos of Balb/C mice]

In recent years, the widespread use of microwave producing instruments specially mobile phones; result in growing concern regarding the possible effects associated with these waves on human health. In the present study investigated the genotoxic effects of mobile phone radiation in adult mice [Balb/C] and their embryos. In this experimental research pregnant mice were irradiated with mobile phone for 4 days during gestational period from 14th to 18th days of gestation for 6h/day from 9AM until 15PM]. At the end of treatment period, euthanized the dams on day 18.5. Then embryos in 18.5th day of gestation were extracted. At first the morphology of embryos was studied, then documented their weight and CR length. For assessment of possible genetic damages in erythrocytes the blood was taken from their hearts and smear was prepared. Spleen tissue was prepared for histological studies. Smear was prepared from peripheral blood and bone marrow of mice and stained with May Grunowald and Gimsa. Data were analyzed using t-test and ANOVA. In experimental group, mobile phone radiation decreased embryos weight [P=0.04] but no change was observed in CR length. Megakaryocytes and red blood cells of spleen were significantly increased [P=0.002 and P<0.05, respectively]. However, lymphocytes.numbers and micronucleus frequency in peripheral blood erythrocytes in experimental embryos and pregnant mice did not change. Interestingly, micronucleus frequency in polychromatic erythrocytes of bone marrow of pregnant mice was significantly increased [P <0.001]. Mobile phone radiation [940 MHZ] had genotoxic effects and increased micronucleus formation in polychromatic erythrocytes of bone marrow in pregnant mice

[Effect of low electromagnetic fields on fetal death and bone marrow megakaryocytes in NMRI mouse neonates]

Low electromagnetic fields [LEMF] are produced by instruments which are works with electricity. This study was done to determine the effect of LEMF on fetal death and bone marrow megakaryocytes in NMRI mouse neonates. In this experimental study 64 females' mice with 6-8 old weeks were used. 2 female mice coupled with one male, and positive vaginal plaque was interpreted as the zero day of pregnancy [GD=0], The pregnant mice were randomly categorized into control and experimental groups. The experimental group were exposed to 50HZ, 0.5 mT Low electromagnetic fields on 7-11 days of pregnant period [8h/d]. The weight of neonate and death fetus were studied after delivery. The live neonates were dissected on 15[th] day, and 1 ml of bone marrow was extracted from Tibia and vertebral column, by pressing method. The bone marrow cells suspended in 1:1 IMDM in 15cc [FULCON] tubule and cells was counted with neobar lam. The data were tested by t-student test; significance was set up at p<0.05. There was significant differences between the mean weight of one day neonate in cases with controls [P<0.05]. The mean of dead fetus in experimental group was higher than controls [P<0.05]. The mean of megakaryocytes numbers higher than controls, but this differences was not significant. This study showed that the number of megakaryocytes and fetal death were increased by low electromagnetic fields exposure during pregnancy